FastQCFastQC Report
Tue 9 Feb 2016
mono6_n5_eco_vitd.prinseq.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n5_eco_vitd.prinseq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18374803
Sequences flagged as poor quality0
Sequence length20-326
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTTT28852.2715858E-516445.861285-289
TTAATGC80352.936749E-511809.909290-294
CTTAACG16354.9879927E-511607.659285-289
CTTTTCG20955.589873E-511323.702280-284
ATTCTTG217500.08725.758295-296
ATCCATA58309.276105E-58138.3037285-289
TCCTTAA83751.9142138E-45665.231285-289
TAATGCT86352.0349075E-45494.651290-294
CCTTAAC52102.4290723E-55203.8726285-289
ATGAAAT191201.6629181E-44963.003290-294
CGTAGAT14253.4910822E-44756.5225275-279
TTTTCGA29553.6140764E-44587.509280-284
TTCTTGG251300.03776.0693295-296
TCGTAGA34700.03646.21275-279
GGTACTC35706.0862524E-43544.0757275-279
GAGTTTA81256.305204E-43336.8833285-289
GATTCAT77207.5895677E-43072.9475280-284
TCGAGTT23750.0010414792755.5024280-284
CGCCGAT12650.00133172132459.4731265-269
TTACCAA100650.00128999662356.995280-284