FastQCFastQC Report
Tue 9 Feb 2016
mono6_n5_vitd.prinseq.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n5_vitd.prinseq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22333423
Sequences flagged as poor quality0
Sequence length20-371
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCGA48555.1284605E-623900.75360-364
AACGTAG19851.1985938E-523383.0310-319
GATCGAT30604.002086E-718960.48360-364
CGATGTA27752.766501E-79292.344360-364
ACAGCTA137255.730376E-58454.509360-364
TCGATCG16254.3695763E-87719.7935340-349
AGCTACG54201.620645E-67136.417360-364
GTCTGAG145151.9226296E-45329.5737350-359
GCTACGT54551.652239E-65317.9717360-364
ATCGATG74005.2431897E-65226.943360-364
TACGTAC24350.05151.8125340-349
ACGTACG27402.0944572E-74578.3447340-349
CTACGTA31050.04152.376350-359
TACGTCT27302.8420254E-74146.811310-319
ATCGATC29553.3404467E-73926.8408320-329
GTACGTC39908.871375E-72837.292310-319
CAGCTAC240501.7963088E-42412.4353360-364
TCGATGT132903.035075E-52182.8093360-364
GCCACTA90050.00162763652147.6614300-309
AAACATC185350.00156726482086.829290-299
Produced by FastQC (version 0.11.3)