FastQCFastQC Report
Tue 9 Feb 2016
mono6_n6_asp.prinseq.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n6_asp.prinseq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16231224
Sequences flagged as poor quality0
Sequence length20-364
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGG459710.2832257135999109No Hit
GACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCC272900.16813272985450758No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAT13453.305886E-514841.138290-294
TACTCGC13853.5054385E-514412.514290-294
TCCTTTG206550.08697.747305
ATTGGCA114151.9843152E-45246.0786295-299
GCGGATT24704.2114247E-44278.447290-294
ACGGGAG52455.0267845E-43805.783290-294
CGGATTG26909.291589E-42903.7007290-294
CGCCGGG378307.2648824E-42374.4646300-304
CCTTATC84950.00131852812349.7742290-294
CAGCCTC392107.804581E-42290.895300-304
TTGGCAG143000.00155677942093.846295-299
AGCCGTA20600.00185421772076.4214285-289
TGACCCG290050.00128107972064.6094295-299
GCATTTA98101.05881554E-72034.7942290-294
TTTCGGG25800.00212811211934.2375280-284
ACGTACT28353.1558797E-41760.2584280-284
CCCGAGC341950.01751.25295-299
CCGAGCC211650.01414.694295-299
AGCCTCC320500.0031279511401.3414300-304
CGTACTG20050.00450986341337.3431280-284
Produced by FastQC (version 0.11.3)