FastQCFastQC Report
Tue 9 Feb 2016
mono6_n6_asp_atra.prinseq.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n6_asp_atra.prinseq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20306333
Sequences flagged as poor quality0
Sequence length20-363
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGG621640.3061310971311265No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCCA64008.81581E-58348.065295-299
TCGGCTA21901.5655336E-46970.3345290-294
CGGCTAA15652.63554E-45462.248290-294
TTTGTAG102752.2722466E-45199.768295-299
TTGTAGG53702.8962188E-44974.638295-299
GGTCGTG39205.015546E-43894.1409290-294
GGGCAGC615050.03474.6843300
CGCCGGT16407.910124E-43178.3235285-289
GCGCCAG88207.8125333E-43028.7764295-299
CCGGTCG9258.9790876E-43000.4978285-289
GTCGTGC30259.84569E-42825.9233290-294
TCGCCGG36950.00102830382754.1785285-289
CGATTAG8950.00107472942744.628275-279
TCGTGCG26750.00127288432496.6174290-294
TCGACCC64100.0013409782381.4404290-294
ATGCTAT67650.00149360372256.4722290-294
GTGTAAC72600.00172014462102.6218290-294
CCGAAAA52600.00208364521934.7316285-289
CCCGGCG54950.00227395381851.9907285-289
TATCTGC91550.00273512821667.3984290-294
Produced by FastQC (version 0.11.3)