FastQCFastQC Report
Tue 9 Feb 2016
mono6_n6_asp_vitd.prinseq.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n6_asp_vitd.prinseq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20749414
Sequences flagged as poor quality0
Sequence length20-355
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGG896930.4322676293412431No Hit
GACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCC491890.23706211654941195No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGCC435100.04841.666300-304
CCGGGCA458150.04598.0767305-307
CTATATC52103.6072807E-44492.661290-294
CCATGAC527950.03990.1675295-299
TCCCGCT59904.7681585E-43907.6401290-294
CGATTTT21058.1124343E-43127.3884280-284
GTAGCGA8500.00126749322528.9421270-274
CGGGCAG485050.02171.5378305-307
CCCGCCG496100.02123.1694300-304
CATGACC526900.01999.0594295-299
CTGACGA59050.00297263541621.591290-294
TTGCCTA67050.0031523721570.9236285-289
CCGCCGG485250.01447.0952300-304
GATTCGA27100.00387825821439.5304275-279
CGTGACA49600.00450290371327.2485280-284
TTAGCAA84701.875378E-91243.5708285-289
TGACCCG428250.01229.7775295-299
ATGACCC590100.00385605781189.9727295-299
AAGCAAA208700.0057862711121.5508290-294
GGGCAGC632500.0044300391110.2023305-307
Produced by FastQC (version 0.11.3)