FastQCFastQC Report
Tue 9 Feb 2016
mono6_n6_atra.prinseq.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n6_atra.prinseq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21979213
Sequences flagged as poor quality0
Sequence length20-365
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTAAG42702.9027724E-514548.363300-304
TTAGTTA41056.706786E-510088.755300-304
TAGTTAA80454.80806E-43860.877300-304
AGCAGAC166304.402638E-43735.5085300-304
TTGGCTA116055.3597364E-43568.6636295-299
CTTAGTT175604.9088E-43537.6714300-304
AGTCTTA57600.00119701382537.6433295-299
GTCTTAG47000.0013536742403.1533295-299
AGCGTAG14650.00166585622202.7927280-284
AGTTAAG114350.00156096882173.0305300-304
GCAGACA224700.00200913291843.0947300-304
TTCAAAA225500.00202346271836.556295-299
TATCCCA80550.0023406971814.6277290-294
CTAAAAA236200.00222002021753.359295-299
GTTAAGA120050.00252315731724.8788300-304
GGTCGTT21400.00355410931507.9867280-284
ACATACA98650.00351056181481.6854290-294
TAACCGT22200.0038247431453.6447280-284
CCATATA101350.00370531581442.2128290-294
CAAGTCT288750.01434.263295-299
Produced by FastQC (version 0.11.3)