FastQCFastQC Report
Tue 9 Feb 2016
mono6_n6_can_atra.prinseq.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n6_can_atra.prinseq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12767608
Sequences flagged as poor quality0
Sequence length20-361
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGG688530.5392787748496038No Hit
GACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCC340940.26703514080319507No Hit
AGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGA327690.2566573159201003No Hit
TGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGA136090.10659005195021651No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAACC50302.0243393E-514224.543330-339
CAAAAGG110100.012997.175350-355
TTAAAAC121450.011782.535340-349
TCCCCCC87356.104824E-58191.122300-309
CCCTTTT106759.117654E-56702.5244320-329
GTTTTTT108909.488621E-56570.1978330-339
TGACGGC43851.5383669E-46526.7456290-299
AAAAGGC110359.7429845E-56483.865350-355
TAAAACC111951.0027567E-46391.197340-349
TTTTTTG114851.05538114E-46229.8174320-329
CCCCCCC60251.7425623E-45937.714300-309
AAAGGCC89851.9377001E-45308.8076350-355
GACGGCG34153.35879E-44655.894290-299
CCCCCGG43704.2777476E-44093.2183300-309
TTTTTTT253100.04038.4631330-339
TTTTTGG88603.7681672E-44037.7795320-329
AAAACCC135504.4067638E-43520.268340-349
ACCCCAA203453.3117895E-43516.8076310-319
CCTTTTT105255.3174107E-43399.0237320-329
TAGGTAC8759.094861E-42973.484270-279
Produced by FastQC (version 0.11.3)