FastQCFastQC Report
Tue 9 Feb 2016
mono6_n6_can_vitd.prinseq.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n6_can_vitd.prinseq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14740105
Sequences flagged as poor quality0
Sequence length20-331
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTGAC111857.800262E-57246.4517300
CAATCCT81002.4543106E-45003.1826295-299
GTCACCG47054.968212E-43828.1528290-294
TTCGGGA50155.644415E-43591.5166290-294
TTACTCA50955.8259175E-43535.1243290-294
ACCAGGA243653.701434E-43326.5571300
GAGGGTG123355.691447E-43285.4302295-299
ACGCGTG11358.3115126E-43104.8289280-284
CCGTTTA13500.0010258732792.474285-289
AGTGATA70300.0011090792562.085290-294
GCCGTTT32050.00147907692299.0088285-289
AATCCTC93650.00153077172163.6829295-299
CCTACAA84350.00159663042135.324290-294
GCACCAC195702.1827873E-102070.8115295-299
AGGGTGG103650.00187509271954.934295-299
CTATTTA92850.00193458321939.8446290-294
TCGGGAG54550.00222572541857.2767290-294
AAGTCTA40250.0023325831830.6395285-289
GTGTCGA20850.00244672061808.0767285-289
GGTGTCG43600.0027369471689.9825285-289
Produced by FastQC (version 0.11.3)