FastQCFastQC Report
Tue 9 Feb 2016
mono6_n6_ctr.prinseq.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n6_ctr.prinseq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23344468
Sequences flagged as poor quality0
Sequence length20-346
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATTG22151.2407363E-657456.64300-302
CGATTGT18555.22116E-634303.625300-302
GATTGTC76951.497349E-46615.54300-302
GGCGTTG42052.0120482E-46053.101285-289
CCGTCCC119800.04249.2974290-294
GCGTTGT37354.797207E-44008.708285-289
GGGGAAA214803.5002368E-43949.921295-299
GTCCCAT72455.972491E-43513.2214290-294
AAAGGGG147955.534923E-43440.7961290-294
TGTCCCA150005.68936E-43393.772290-294
TAACGGT9907.4417016E-43296.2043275-279
TGCGCGG39458.263427E-43072.399280-284
GTCGGCC86708.5527136E-42935.7888285-289
AGTTGGT194459.560523E-42617.9778290-294
CGTTGTC42850.00117449712582.648285-289
AAGGGGG104650.0012460282432.2302290-294
TTCGCGA10404.4838816E-72399.4429265-269
AGGGGGG75950.00132716632393.8013290-294
TTAACGG27800.02233.1365275-279
CGGCCGT50453.5922367E-62193.5874285-289
Produced by FastQC (version 0.11.3)