FastQCFastQC Report
Tue 9 Feb 2016
mono6_n6_eco.prinseq.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n6_eco.prinseq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13116633
Sequences flagged as poor quality0
Sequence length20-337
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGCG6054.0103987E-7101061.65295-299
TAGGCGC6552.8203776E-646673.51295-299
GTTGTAC39801.7355716E-515362.388300-301
AGCAATT50152.7556134E-512191.885290-294
CAATTAG18905.870476E-510783.476290-294
TATAAGT57403.6099416E-510651.969295-299
CGTCGAC6508.794862E-59406.508265-269
CGCTGGC70155.3917753E-58715.9375300-301
CTTTCTA80407.082531E-57604.764290-294
AGGCGCT33350.06111.1743295-299
AATTAGG25652.0182396E-45959.2886290-294
ATAAGTT53801.9026845E-45682.3696295-299
TCAAATC55952.0577913E-45464.0127285-289
TTGTACC75751.8860142E-45381.0605300-301
ATCGACT18452.9088123E-45098.3784270-274
ATTAGGC24102.863369E-45074.05290-294
AGTATTC34602.754273E-45048.91275-279
GTCAAGC36554.0978618E-44182.0996285-289
TCCAACC48753.9054465E-44180.6704280-284
TAAGTTG49003.9456034E-44159.34295-299
Produced by FastQC (version 0.11.3)