FastQCFastQC Report
Tue 9 Feb 2016
mono6_n6_eco_atra.prinseq.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n6_eco_atra.prinseq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14716030
Sequences flagged as poor quality0
Sequence length20-328
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTACGT31902.000932E-516520.77290-294
CTACGTA16402.6442496E-516067.455290-294
ACTCGCT34052.279737E-515477.607290-294
TACGTAC12101.3691988E-514518.251290-294
ACGTACG9754.112168E-513513.143290-294
GAGACTA65300.08070.6357295-296
CGATGTA16251.349908E-47484.2017280-284
GTACAGC58901.3642559E-46710.686285-289
AGAGACT134550.05875.279295-296
TCGCTCT31052.2746858E-45657.6763290-294
AATATCG12152.902406E-45205.0625275-279
CACTCGC19904.7565365E-54413.8403285-289
CCACTCG25104.3352338E-44199.3027285-289
CTATGTC54904.1481003E-44114.0713280-284
TCGATGT58454.7018667E-43864.2007280-284
CGCGGCG33005.56662E-43685.4023275-279
TATCGAT10106.61811E-43478.6306275-279
TCCCCTT120655.7239685E-43276.083285-289
CGTAGTC11557.5269333E-43259.1992265-269
ATATCGA14107.751853E-43203.7236275-279
Produced by FastQC (version 0.11.3)