FastQCFastQC Report
Tue 9 Feb 2016
mono6_n6_eco_vitd.prinseq.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n6_eco_vitd.prinseq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14302232
Sequences flagged as poor quality0
Sequence length20-335
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGG380860.2662941001096892No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGAAC87000.018290.35290-292
TGGAACG51300.015509.36290-292
GGAACGT48100.011027.445290-292
ATCTAGC26504.1344017E-44289.112280-284
GCTGTAT72355.0798757E-43665.6543285-289
GGTGCGT10758.7916374E-43020.9023275-279
AGTTTGC91258.0803107E-42906.4116285-289
ATGCGCG7350.0012772832517.4187270-274
TCGTGTA17300.00191659122044.0083270-274
GGGTGCG32800.01940.5614275-279
CTGTATT75250.0020146051922.3925285-289
GTATTAT49250.00106586761794.9921285-289
ATGTAGG36800.00262784861729.6307275-279
GAAAATA154100.0023042091721.0258285-289
GGATTTT154800.00232518721713.2433285-289
TCTAGCT35850.0029178291643.9489280-284
CTAGTAT22800.00332861771550.936270-274
GCGCGTA7050.00368904371484.9388270-274
CATGCGC24100.00371893121467.2756270-274
CGTGGCC79250.00369661631434.2139280-284
Produced by FastQC (version 0.11.3)