FastQCFastQC Report
Tue 9 Feb 2016
mono6_n6_vitd.prinseq.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n6_vitd.prinseq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13206378
Sequences flagged as poor quality0
Sequence length20-335
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGAGGTAGTGA236120.17879239864253468No Hit
TGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCC218610.16553365351196217No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAGA32102.0243178E-516425.064300-304
TGGACCG143500.011022.534310-311
ATAGGTT21300.07425.979290-294
TCGAGAG48151.5181664E-46570.0254300-304
ACGTCGA8351.9859699E-46314.306280-284
GGACCGG126100.06271.743310-311
ATTCTTG135101.1952643E-45853.9365305-309
CGAGAGT40352.238838E-45600.0483300-304
GAGTGGA171301.9216222E-44616.852305-309
CGGCAGT67702.9394263E-53893.9773295-299
AGGGTCC75955.6655816E-43470.9976295-299
AGTGGAC120650.03277.5251305-309
GTGAAAT162505.1874213E-43244.5818300-304
CAGTCGA34001.00715624E-73101.4385295-299
ATAGGGG87907.5885345E-42999.1155295-299
GTGGACG88707.727283E-42972.0662305-309
AGTCGAG31804.1985913E-82763.3362295-299
CGATGAG57709.809185E-42741.3062290-294
TAGGTTT31351.4588295E-92655.4749290-294
GCAGTCG52152.2524546E-82527.5386295-299
Produced by FastQC (version 0.11.3)