FastQCFastQC Report
Mon 21 Nov 2016
N1_24h_IFN_RNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameN1_24h_IFN_RNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69072465
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC1696410.24559858982881239TruSeq Adapter, Index 4 (100% over 51bp)
GGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTA1419460.20550301773651775No Hit
GTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCGTCG1335730.19338096591746073No Hit
GTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGCTTGTG870880.1260820791613561No Hit
GTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTT790700.1144739803335526No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG226750.037.01452343
CGTATGC226750.036.8255844
GTATGCC240100.034.9361945
CGTCTGA250100.033.78577416
ACACGTC272550.031.21983313
GATCGGA279700.030.60011
ATCGGAA283100.030.2049642
GACCAAT282500.030.07580635
TCTCGTA284450.029.62460141
CACACGT294900.028.99016212
ACCAATC293150.028.82998736
ACGTCTG305700.027.87708515
CACGTCT313400.027.13633714
ATCTCGT313950.026.87687340
GCGTACG98050.026.0010382
TCGGAAG330650.025.9764393
TGACCAA328250.025.93828234
ACTGACC338150.025.19210432
CGTACGG101150.025.1598743
GCACACG348050.024.81412111