FastQCFastQC Report
Mon 21 Nov 2016
N1_24h_RVFV_RNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameN1_24h_RVFV_RNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70118384
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTG1076160.15347758157118965No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTG1041030.14846748322094816No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC975160.13907337054430688TruSeq Adapter, Index 5 (100% over 51bp)
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG878700.12531663593388004No Hit
GTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCGTCG717040.10226134133382195No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG144850.033.0321943
GTATGCC156700.030.47555745
CGTATGC161850.029.33989544
CGTCTGA170750.028.42471916
ACACGTC185000.026.30882313
GATCGGA187050.026.0114881
ATCGGAA190950.025.4876612
ACGTCTG209250.023.30199815
TCTCGTA208750.023.0175341
GCGTACG82550.022.9637642
CACACGT213250.022.88630512
AGTGATC214400.022.54723436
CGTACGG84600.022.3543113
CACGTCT219950.022.11834314
ATCTCGT219550.022.0078240
TCGGAAG237300.020.7737143
GCACACG258900.019.11916711
CTAATAG188500.018.55000118
CGGTTCG59100.018.0092373
GATCTCG272000.017.97027239