FastQCFastQC Report
Mon 21 Nov 2016
N1_24h_mock_RNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameN1_24h_mock_RNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68994759
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTG1174040.17016364967663702No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTG793100.11495076024542676No Hit
GTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCGTCG755890.10955759697631524No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG86650.024.9184743
CGTATGC87950.024.49827444
GCGTACG72400.022.48062
GTATGCC98150.022.31769645
CGTACGG76000.021.3862153
CGTCTGA116800.019.07727416
ACTTAGG116900.018.60504332
CTAATAG209350.018.19156818
AGCGTTG324300.017.919845
ACACGTC129150.017.1515713
CACTTAG128350.016.98084431
TAATAGG227150.016.79094519
AACGTGA230050.016.68200527
GTACGGA98150.016.6740764
ACGTGAG232700.016.55856728
TACGGAA100450.016.4261785
ATCGGAA136950.015.9934592
GAACGTG241250.015.97254826
GATCGGA137450.015.9319511
ATGTCGG373600.015.9016876