FastQCFastQC Report
Mon 21 Nov 2016
N1_6h_IFN_RNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameN1_6h_IFN_RNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70103253
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTG970660.1384614776720846No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG925100.13196249252513287No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTG891000.127098238936216No Hit
CCCCTCCTTAGGCAACCTGGTGGCCCCCCGCTCCCGGGAGGTCACCATATT760780.10852278138933154No Hit
CCTTAGGCAACCTGGTGGCCCCCCGCTCCCGGGAGGTCACCATATTGATGC723180.10315926423556977No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTACG69250.022.0432072
TCGTATG74950.021.29730243
CGTACGG71000.021.0894663
CGTATGC74950.021.02708844
ACCGATG77250.020.57667432
TATCTCG80900.019.81445539
GTATGCC82100.019.25029645
CACCGAT84600.018.89529631
TCACCGA89600.017.79069930
AGCGTTG291000.017.19462245
CTAATAG188250.017.115918
GTACGGA91000.016.5036834
TACGGAA91300.016.400355
AACGTGA200500.016.21722627
CGTCTGA102650.015.71665916
ATGGACC276900.015.70719942
ACGTGAG211350.015.38382128
TAATAGG213700.015.20938619
CTACGTT286250.015.02782426
GAACGTG217050.014.99089826