FastQCFastQC Report
Mon 21 Nov 2016
N2_24h_IFN_RNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameN2_24h_IFN_RNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66989439
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTA1604180.23946759727305672No Hit
GTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCGTCG1573120.2348310455324159No Hit
CTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTG1435510.21428900158426464No Hit
GTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGCTTGTG925740.13819193201483596No Hit
GTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTT821670.12265664741572176No Hit
GCCCTTCGTCCTGGGAAACGGGGCGCGGCCGGAAAGGCGGCCGCCCCCTCG811570.12114894707507552No Hit
GGGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCT771110.11510918907680359No Hit
CCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGGCAA743390.1109712233894062No Hit
GTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGAC735420.10978148361564873No Hit
AGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGCTTCCAT718670.10728108948635919No Hit
GTCGCTTTTTGATCCTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAA686400.10246391225936374No Hit
GCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATGT685470.10232508440621514No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC96400.031.63538244
TCGTATG102900.029.94310243
AGTCACG110300.027.88072828
GTATGCC111050.027.74422345
GCGTACG100050.025.775982
CGTACGG106850.024.1775193
CTAATAG323450.023.7265818
CGTCTGA132950.023.3761316
TAATAGG339600.022.5870619
TAGGGAA354350.021.79117222
ACGGCTA140650.021.76428632
ACTAATA354800.021.70764717
ACACGTC143700.021.67433513
GCTACAT146850.021.31857735
AATAGGG366600.021.01733420
ATAGGGA368100.020.97671721
TCTCGTA147100.020.90034941
AACGTGA371300.020.73410627
GGCTACA151500.020.66410434
CCACTAA380050.020.3338615