FastQCFastQC Report
Mon 21 Nov 2016
N2_6h_IFN_RNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameN2_6h_IFN_RNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69501986
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTA2173800.31276804090173765No Hit
GTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCGTCG1988580.2861184427161549No Hit
GTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGCTTGTG1320810.19003917384461502No Hit
GTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTT1217740.17520938178658665No Hit
GCCCTTCGTCCTGGGAAACGGGGCGCGGCCGGAAAGGCGGCCGCCCCCTCG1028570.14799145451757306No Hit
CTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTG1007970.14502751043689602No Hit
GTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGAC975160.140306782024905No Hit
GCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATGT970650.1396578797043296No Hit
GGGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCT943080.13569108658276327No Hit
GGTGTATGTGCTTGGCTGAGGAGCCAATGGGGCGAAGCTACCATCTGTGGG870820.1252942613754951No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC861170.12390581184255654TruSeq Adapter, Index 10 (100% over 51bp)
GTCGCTTTTTGATCCTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAA822880.11839661675279321No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAA790180.11369171522666993No Hit
GCCGAAACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGG776740.11175795753519907No Hit
GGCGTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCT776710.1117536411117806No Hit
AGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGCTTCCAT759110.10922133937294973No Hit
GGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGTCCGTAGCGGTCCTGAC714330.10277835801699249No Hit
GCTTGATCTTGATTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCAC712000.10244311579815862No Hit
CCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGGCAA697240.10031943547627545No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC123100.034.26892544
TCGTATG129700.032.8890143
TATCTCG129700.032.3196839
GTATGCC130700.032.1901445
TAGCTTA136000.031.2184234
ACTAGCT144600.029.39247332
CACTAGC147800.028.65079731
CGTCTGA154400.027.65442816
GCGTACG145650.027.1290252
GCTTATC156900.026.87413236
GTCACTA166100.025.49045829
CGTACGG154200.025.4649073
ACACGTC168300.025.45059813
CTAGCTT171850.024.91437133
AGCTTAT172450.024.78918835
CTAATAG406650.024.15823218
TCACTAG176450.023.98580230
TAATAGG418350.023.57636819
TCTCGTA181950.023.4323841
CACACGT187000.023.08484612