FastQCFastQC Report
Mon 21 Nov 2016
N2_6h_RVFV_RNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameN2_6h_RVFV_RNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70292881
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTG1809020.25735465302666993No Hit
GTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCGTCG1754860.24964974760388609No Hit
GGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTA1469660.20907664888568162No Hit
GTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGCTTGTG968140.1377294522897703No Hit
CCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGGCAA817110.1162436349706594No Hit
GTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTT803160.1142590812290081No Hit
GTCGCTTTTTGATCCTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAA779920.1109529142787589No Hit
GCCCTTCGTCCTGGGAAACGGGGCGCGGCCGGAAAGGCGGCCGCCCCCTCG752520.10705493775393841No Hit
CTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAAC752370.10703359846639378No Hit
CTTCGTCCTGGGAAACGGGGCGCGGCCGGAAAGGCGGCCGCCCCCTCGCCC718030.10214832423784138No Hit
GGCGTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCT711020.10115106819992199No Hit
GGGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCT708930.1008537407934667No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC94700.029.59213444
TCGTATG98150.028.98720743
GTATGCC104250.026.9670145
AGTCACG105800.026.66023428
GCGTACG144650.026.4998022
CGTACGG150650.025.3402063
GTCACGA115300.024.32804129
CTAATAG367900.023.75166518
TAATAGG376350.023.20270319
CGTCTGA121400.023.08065216
TAGGGAA387050.022.56914322
ACTAATA398100.022.0015417
ATAGGGA407300.021.48034521
AACGTGA410950.021.25876227
AATAGGG411600.021.17622420
GGCGTAC191400.020.9100381
ACACGTC137000.020.83067713
ACGTGAG422350.020.720828
GAACGTG423450.020.6522826
CCACTAA428150.020.5502915