FastQCFastQC Report
Wed 23 Nov 2016
N1_24h_RVFV_RNA.prinseq.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameN1_24h_RVFV_RNA.prinseq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70092138
Sequences flagged as poor quality0
Sequence length15-51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTG1027930.14665410833951165No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTG1008360.14386206909539556No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC911440.13003455537338582TruSeq Adapter, Index 5 (100% over 51bp)
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG824090.11757238736247423No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG138150.033.12296343
GTATGCC145900.031.77066245
CGTATGC152150.029.90165744
CGTCTGA163350.027.6196916
GATCGGA180050.025.1980781
ATCGGAA180800.025.0935522
ACACGTC183950.024.50050513
TCTCGTA203250.022.55860741
CACACGT207750.021.84893212
AGTGATC211450.021.741236
GCGTACG82050.021.6756612
ACGTCTG209450.021.5528615
CGTACGG82700.021.4785483
ATCTCGT215950.021.35692240
CACGTCT218950.020.79260314
TCGGAAG229000.020.0147043
GCACACG253150.018.25018911
CTAATAG194000.018.09994118
AGCGTTG286550.018.00096545
CGGTTCG58350.017.4007023