FastQCFastQC Report
Wed 23 Nov 2016
N1_24h_mock_RNA.prinseq.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameN1_24h_mock_RNA.prinseq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68967128
Sequences flagged as poor quality0
Sequence length15-51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTG1119230.1622845596818241No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTG765560.11100360739974557No Hit
GTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCGTCG727320.10545893690106974No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC83750.024.68941944
TCGTATG84600.024.61936643
GTATGCC91950.023.48042545
GCGTACG70250.021.18862
CGTACGG73100.020.5440753
AGCGTTG311850.018.415345
CTAATAG208350.017.97352818
CGTCTGA115850.017.88707216
ACTTAGG119000.017.59882432
AACGTGA221600.016.94485527
ACGTGAG224750.016.78060228
TAATAGG226200.016.70764219
ATGGACC249800.016.64148542
CACTTAG127400.016.45381531
ACACGTC130200.016.28558513
GAACGTG233800.016.10417626
GATCGGA130250.015.9618671
GTACGGA96050.015.819534
CTACGTT253500.015.79417926
TAGGGAA241200.015.71503822