FastQCFastQC Report
Wed 23 Nov 2016
N1_6h_IFN_RNA.prinseq.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameN1_6h_IFN_RNA.prinseq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70076333
Sequences flagged as poor quality0
Sequence length15-51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTG926810.13225720586720768No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG869130.12402618156403818No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTG862740.12311431878149216No Hit
CCCCTCCTTAGGCAACCTGGTGGCCCCCCGCTCCCGGGAGGTCACCATATT734350.10479286922733243No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC70050.021.49702544
GCGTACG66150.021.3713072
TCGTATG73700.021.2124843
ACCGATG74100.020.877232
CGTACGG69550.020.3583973
TATCTCG80600.019.64925639
GTATGCC80850.019.25573745
CACCGAT85850.017.99787931
AGCGTTG279500.017.18730545
TCACCGA91500.017.18660230
CTAATAG186350.016.98010818
ATGGACC277300.016.58254442
GTACGGA87400.016.276474
AACGTGA198150.016.11144427
TACGGAA89350.015.6489355
CGTCTGA102600.015.45174216
TAATAGG209100.015.40288619
CTACGTT289050.015.1881726
ACGTGAG212550.015.13683928
AATGGAC305700.015.07726641