FastQCFastQC Report
Wed 23 Nov 2016
N1_6h_RVFV_RNA.prinseq.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameN1_6h_RVFV_RNA.prinseq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68545199
Sequences flagged as poor quality0
Sequence length15-51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCGTCG2129220.31063007053200037No Hit
GGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTA2035560.2969660938616576No Hit
GTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGCTTGTG1816530.2650119959532104No Hit
GTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTT1122780.16380140642672875No Hit
GCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATGT958110.13977784206301597No Hit
GGGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCT937410.13675793690525284No Hit
GTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGAC880290.12842474933948328No Hit
GCTTGATCTTGATTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCAC845680.12337552627135855No Hit
GTCGCTTTTTGATCCTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAA806460.11765375427679478No Hit
CCTTAGGCAACCTGGTGGCCCCCCGCTCCCGGGAGGTCACCATATTGATGC791930.11553398510083836No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC102400.031.97380644
TCGTATG105900.031.23509643
GTATGCC113200.029.55701645
TATCTCG113750.028.50640539
AGTCACG118250.027.61347628
ACGCCAA124700.026.47826432
CGCCAAT125850.026.07945333
GCGTACG123200.025.4261052
CGTCTGA132900.024.59903716
CGTACGG127950.024.4649413
CTAATAG446150.023.55255718
TAATAGG455350.023.10607519
TAACGAG76300.022.79469118
TAGGGAA464650.022.68215822
CACGCCA148300.022.52464131
GCCAATA148250.022.25635534
AAGCCCG174250.022.22357644
ACACGTC146700.022.1690513
TTAACGA78650.022.10296417
ACTAATA477550.022.06452617