FastQCFastQC Report
Wed 23 Nov 2016
N2_24h_IFN_RNA.prinseq.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameN2_24h_IFN_RNA.prinseq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66958823
Sequences flagged as poor quality0
Sequence length15-51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTA1535670.22934542920505038No Hit
GTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCGTCG1515330.22630774140100998No Hit
CTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTG1371520.2048303626842425No Hit
GTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGCTTGTG871280.13012176154888505No Hit
GTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTT794620.11867293425991075No Hit
GGGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCT732540.10940156460038135No Hit
GTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGAC708480.10580831147524801No Hit
CCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGGCAA707440.10565299213816826No Hit
AGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGCTTCCAT682970.10199850735130155No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC84300.031.17546844
TCGTATG89500.029.84279343
GTATGCC98350.027.29854245
AGTCACG104000.026.84067528
GCGTACG98250.025.4963552
CGTACGG102700.024.3915943
CTAATAG325000.023.1666218
TAATAGG342900.022.01772719
TAGGGAA346850.021.86436522
CGTCTGA128850.021.72205416
ATAGGGA361850.020.96957221
ACTAATA362000.020.8566517
AATAGGG363500.020.7964120
AACGTGA366250.020.61262327
ACACGTC136950.020.46840113
ACGTGAG373650.020.24860628
GCTACAT141250.020.1121935
ACGGCTA137400.020.10702732
GAACGTG375900.020.09969726
CCACTAA380650.019.92565715