FastQCFastQC Report
Wed 23 Nov 2016
N2_6h_IFN_RNA.prinseq.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameN2_6h_IFN_RNA.prinseq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69467112
Sequences flagged as poor quality0
Sequence length15-51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTA2080560.29950287842684464No Hit
GTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCGTCG1918020.276104755873542No Hit
GTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGCTTGTG1241810.17876228970048447No Hit
GTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTT1178460.16964286639697934No Hit
CTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTG965040.13892041459849375No Hit
GTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGAC939210.13520210830126347No Hit
GCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATGT899720.12951740386155683No Hit
GGGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCT897270.12916471898241574No Hit
GCCCTTCGTCCTGGGAAACGGGGCGCGGCCGGAAAGGCGGCCGCCCCCTCG821810.1183020247048704No Hit
GGTGTATGTGCTTGGCTGAGGAGCCAATGGGGCGAAGCTACCATCTGTGGG799720.11512210267212492No Hit
GTCGCTTTTTGATCCTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAA797970.11487018490130986No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC795250.11447863270895729TruSeq Adapter, Index 10 (100% over 51bp)
GCCGAAACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGG726640.10460201656288806No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAA724820.10434002208124041No Hit
AGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGCTTCCAT721500.10386209808175126No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC117800.035.05216244
TCGTATG122250.033.92987443
GTATGCC129150.032.6145
TATCTCG128350.031.95265239
TAGCTTA136650.029.86648234
ACTAGCT145600.028.01041632
GCGTACG143500.027.6554452
CACTAGC149500.027.2111231
CGTCTGA155000.026.2258716
GCTTATC158650.025.80609136
CGTACGG156550.025.2795943
GTCACTA166000.024.7920629
CTAGCTT166050.024.68031133
ACACGTC170750.024.01320513
AGCTTAT171800.023.96003335
CTAATAG406150.023.42775218
TCACTAG175550.023.42437430
TCTCGTA180250.023.14740641
TAATAGG417050.022.87705419
AAGCCCG195300.022.61592144