FastQCFastQC Report
Wed 23 Nov 2016
N3_6h_IFN_RNA.prinseq.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameN3_6h_IFN_RNA.prinseq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70331915
Sequences flagged as poor quality0
Sequence length15-51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTG1758150.2499789746944897No Hit
GGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTA1637150.23277483628876025No Hit
GTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCGTCG1304160.18542933176211682No Hit
CCGAAAGATGGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCTG953390.1355558141705654No Hit
AGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGCTTCCAT942030.13394061572189525No Hit
GTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTT877950.12482953151496017No Hit
GGGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCT830720.11811423021824445No Hit
CTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGC816450.11608527935006463No Hit
CAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAG808060.11489236429862602No Hit
CCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGGCAA783150.11135058671443825No Hit
GTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGCTTGTG782230.1112197783893699No Hit
CTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAAC760180.10808464407659026No Hit
CTCTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAG746490.1061381593263883No Hit
GCCCTTCGTCCTGGGAAACGGGGCGCGGCCGGAAAGGCGGCCGCCCCCTCG707840.10064278784389705No Hit
GTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGAC707510.10059586746642686No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG91450.031.51746245
CCGTATC97050.031.16425138
TATCTCG105050.028.6238741
GCGTACG150550.026.6479532
GTTCCGT114400.026.60037835
CGTACGG155500.025.8422893
TCCGTAT123100.025.17236137
CGTCTGA129250.024.00835816
CGTATCT125950.023.96168739
TTCCGTA133900.023.23637236
GTATCTC135400.022.5571840
AGTTCCG147700.022.16311634
ACACGTC143050.021.90432413
GGCGTAC191250.021.7043151
GTACGGA189950.021.2019374
TACGGAA192650.020.8475445
TCTCGTA144550.020.7353343
CTAATAG310400.020.56026818
AAGCCCG198800.020.34578744
TAATAGG323300.019.8530619