FastQCFastQC Report
Wed 7 Dec 2016
15_230_blood.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename15_230_blood.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37026491
Sequences flagged as poor quality0
Sequence length50
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGN716100.19340206988558542No Hit
CTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGAGAATTAATGTN638000.17230906379975353No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCGA79450.056.79541844
TAATGTG137050.031.08942644
GCGTTCG160050.028.53515843
AGTGCGT173150.026.08886140
TGCGTTC182800.024.73536942
TCGTGCG164100.024.3071047
GTGCGTT186600.024.23164741
CTCGTAT32650.024.11940242
CGTCGAT167000.023.39282812
GCGTCGA168300.023.31495711
CGTGCGT170900.022.9602228
CGGCTCG171450.022.9553243
GGCTCGT176900.022.3535964
AGGCGTA208350.022.0828388
GCGTAGC218300.021.38351810
TGCGAGA185700.021.0513535
TAGCCCC219750.020.96677413
AAGTGCG216850.020.94113339
GTGCGTC188850.020.8351489
TCTCGTA38750.020.54586641
Produced by FastQC (version 0.11.3)