FastQCFastQC Report
Wed 7 Dec 2016
15_231_blood.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename15_231_blood.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36201291
Sequences flagged as poor quality0
Sequence length50
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCGA19450.055.55163244
CTCGTAT41650.030.45530742
TATCTCG44750.028.10397539
TCTCGTA47950.026.54385641
CGTATGC42450.026.16997144
GCGTTCG42300.024.88746643
TCGTATG53750.024.20137843
CGATGTA57150.022.23222434
ACACGTC57800.022.16575213
CGTCGAT45100.022.0947912
CCGATGT58500.021.79292333
TCGTGCG49650.021.5470057
GATCGGA57650.021.5302981
GCGTCGA47500.021.16005311
CACCGAT62550.020.8965531
AGTGCGT50700.020.50837940
CGTGCGT49650.020.4175248
ATCTCGT63200.019.93370440
ACGTCTG69600.018.62718815
TGCGAGA53350.018.59975435
Produced by FastQC (version 0.11.3)