FastQCFastQC Report
Wed 7 Dec 2016
15_232_blood.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename15_232_blood.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41360880
Sequences flagged as poor quality0
Sequence length50
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT21400.034.88376244
ACCCGTC36350.019.50317432
TCTCGTA38500.018.35893843
CGTCCCG42850.016.54535
TCGCAAC65200.015.48179940
ACACGTC50750.014.26559913
CCGATCT51250.013.66493139
CACCCGT54550.013.589622531
ATCTCGT53850.013.36649442
TTCGCAA76100.013.32123639
CGACGGA29450.013.20937115
CGTGGAT59150.012.6482981
TAAGTCG26150.012.64490920
CGCAACA81500.012.49172141
CGACGAT23200.012.48899651
GCATTCG89300.012.44100336
GATCGGA48650.012.4002971
ATTCGCA84050.012.08694338
CATTCGC83200.012.08052937
GATCTCG59650.012.03058141
Produced by FastQC (version 0.11.3)