FastQCFastQC Report
Wed 7 Dec 2016
15_233_blood.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename15_233_blood.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36595427
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCAAC65150.017.82773840
CTCGTAT30650.016.2309942
TTCGCAA78350.014.90816539
CGCAACA80500.014.48314841
CGACGGA30100.014.26829715
TCTCGTA35750.014.09934841
ATTCGCA83900.013.8698438
GCATTCG93350.013.31070736
CATTCGC87950.013.231132537
CGACGAT21350.012.8379491
CTCGAAT33400.012.6705181
TCGTATG41600.012.59079143
CGCAATT12350.012.25083351
AACGACG38750.011.76137713
CGCGTTT3755.456968E-1211.6945151
ACGGACT38100.011.32988717
ACGACGG39750.011.07990714
GACGGAC42450.010.891520516
CGATCTA4650.010.8386514
GTAAGGT50550.010.7931818
Produced by FastQC (version 0.11.3)