FastQCFastQC Report
Wed 7 Dec 2016
15_234_blood.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename15_234_blood.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37587387
Sequences flagged as poor quality0
Sequence length50
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT35700.025.95644642
TCTCGTA43500.021.84973141
TCGTATG56300.017.57416243
CGTATGC55050.017.53849844
GATCGGA53500.017.2083151
GATCTCG54100.017.20827939
ACACGTC55600.017.01397913
ATCTCGT54750.016.88534740
TCGCAAC69100.016.22997940
ACGTCTG65400.014.29917515
TTCGCAA81200.013.86479839
CACACGT70000.013.85412812
CACGTCT67850.013.81462114
CGCAACA82900.013.71120641
GTAAGGT53000.013.3149598
CGACGAT19100.013.156121
ATTCGCA87450.012.9482338
GCATTCG93750.012.93230836
CATTCGC87350.012.91348237
CGACGGA25950.012.84660415
Produced by FastQC (version 0.11.3)