FastQCFastQC Report
Wed 7 Dec 2016
24_1055_blood.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename24_1055_blood.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36301042
Sequences flagged as poor quality0
Sequence length50
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT19800.045.87492844
TATCTCG36850.025.2546941
TCTCGTA42000.022.10669143
GTTCCGT41350.021.98431635
CCGTATC42200.021.64461138
TCCGTAT45200.020.20782737
TTCCGTA45300.020.1625536
CGTATCT47750.019.21889539
ACACGTC52750.018.00652713
GATCGGA55950.016.3741951
ATCTCGT56750.016.32293942
CACACGT64700.014.98039412
TCGCAAC40450.014.69882940
ACGTCTG64700.014.6146115
CACGTCT64900.014.56929114
AGTTCCG72050.013.09532234
CGACGAT23600.012.8788831
GCACACG80600.012.31915511
CTCGAAT43550.012.1268781
TAAGTCG39650.011.73376920
Produced by FastQC (version 0.11.3)