FastQCFastQC Report
Wed 7 Dec 2016
24_1056_blood.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename24_1056_blood.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36350858
Sequences flagged as poor quality0
Sequence length50
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGN370330.1018765499290278No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCGA36000.059.20883644
GCGTTCG81550.027.25535643
CTCGTAT39900.026.09370842
TCTCGTA43650.024.14882941
AGTGCGT90450.024.04760440
TCGTGCG86750.022.8789167
CGTCGAT83700.022.86112612
CGTGCGT85450.022.3929378
GTGCGTT99600.021.92564241
GCGTCGA87600.021.76937711
CGGCTCG90150.021.471213
TGCGTTC103800.021.03853442
ACACGTC50100.020.6051613
GGCTCGT93500.020.5343384
TGCGAGA93150.020.45142235
TAATGTG103600.020.36347844
AGGCGTA112400.020.059668
GCGTAGC114450.019.9456710
TAGCCCC118850.018.9345213
TCGTATG58050.018.828243
Produced by FastQC (version 0.11.3)