FastQCFastQC Report
Wed 7 Dec 2016
24_1057_blood.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename24_1057_blood.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37279174
Sequences flagged as poor quality0
Sequence length50
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT28750.022.85133442
TCTCGTA34850.019.3492641
ACACGTC41800.016.23290313
TCGCAAC48700.016.07227940
CGACGAT18350.015.6046011
GATCGGA41450.014.8107371
TCGTATG49950.014.54165343
GATCTCG46150.014.47045839
ATCTCGT48000.014.13846640
ACGATCT46200.014.12626137
CGTATGC54850.014.02193144
GTAAGGT64200.013.236798
CTCGAAT33950.013.2265131
ACGTCTG53000.013.13023315
CGCTTAT42050.012.9938538
ATCACGA84700.012.87573734
TAAGGTG67650.012.7540319
CACACGT57400.012.53886912
CGCAACA63150.012.5321741
ATTCGCA63400.012.5168538
Produced by FastQC (version 0.11.3)