FastQCFastQC Report
Wed 7 Dec 2016
24_1058_blood.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename24_1058_blood.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37921150
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCAAC97050.018.18254940
CGCAACA105100.016.91528941
CTCGTAT23750.016.70629942
TTCGCAA112800.015.76054839
CATTCGC113050.015.74512837
ATTCGCA116550.015.29112938
GCATTCG125100.015.13945636
CGACGGA33750.014.02750915
CGACGAT13200.013.6257231
TATTGCG73750.013.402987
CTCGAAT30900.013.132061
CGTGGAT77050.013.094981
TCTCGTA31550.013.06230341
GTTCGTC26000.012.9917321
ACGACGG36850.012.90673814
TGCGAGT79850.012.48889210
TCGTATG33800.012.45225743
ATTCGTC48050.012.2244196
GATTCGT47200.012.2132575
AATCTCG33600.011.93909439
Produced by FastQC (version 0.11.3)