FastQCFastQC Report
Wed 7 Dec 2016
24_1059_blood.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename24_1059_blood.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38410820
Sequences flagged as poor quality0
Sequence length50
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACATCAAAGTGAGGGAAGTAGGTCTTGGTGGTGGGGAAGCTAGCAAN684220.17813209923662135No Hit
CTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAN425650.1108151296952265No Hit
CAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGN404050.10519171420969405No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCGA11650.023.48495544
TCGCAAC71550.018.00302940
CTCGTAT22750.016.78731542
CGTCGAT15700.016.21419512
CGCGTTT3650.015.9673061
TTCGCAA83300.015.7226339
CGCAACA84350.015.50147241
TATCTCG25150.015.09927639
TAGCAAA232750.014.91040344
CATTCGC87100.014.78909437
ATTCGCA88800.014.60316338
GCATTCG94450.014.55168536
CTAGCAA266200.014.37909643
TCGTGCG22650.014.0963657
AAGCTAG280550.013.78188640
CGACGAT17150.013.7190211
CGACGGA28050.013.69025415
AGCTAGC281700.013.58023641
GCGTTCG18650.013.53608243
GCGTCGA19150.013.40575411
Produced by FastQC (version 0.11.3)