FastQCFastQC Report
Thu 8 Dec 2016
24_190214_1HS_ACTGAT_L001_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename24_190214_1HS_ACTGAT_L001_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58486873
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAT73100.013.2521261
CTCGTTT92800.011.6527321
CTCGGTT79900.09.7276251
CGAATGT68350.09.6441393
CTCGGAT110200.09.6083931
CCGGAAT69650.09.5742571
TCGAATG68550.09.520062
CGCAATT40350.09.2682781
CTCCGAT84200.09.1505781
CGGAAAT71800.09.0365481
CTCAAAT210150.08.9406921
CTGGAAT285250.08.7903061
CAACGAT60700.08.7590661
CTCGATT47450.08.6411111
CGCAAAT36500.08.579391
CGACGAT30400.08.5223371
TCGGTTA34150.08.5005722
GTCGGTT57700.08.3554991
CTTAGAT137750.08.2427741
CTCGACT48450.08.1837691
Produced by FastQC (version 0.11.3)