FastQCFastQC Report
Thu 8 Dec 2016
24_190214_3HS_CTTGTA_L003_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename24_190214_3HS_CTTGTA_L003_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45398854
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAT58200.013.3441751
CTCCGAT66150.010.4776741
CTCGTTT69250.010.0738381
TCGAATG52650.09.81458952
CTCGGAT84350.09.63548851
CTCGGTT63450.09.5714321
CGAATGT52600.09.5368753
CTCAAAT152300.09.3389261
CGACGAT26550.09.0135861
CTCAGAT196700.08.83776951
CCGGAAT55900.08.8044251
CTGGAAT222000.08.6949971
CTCGCAT35700.08.6005651
CGCAATT28150.08.5012691
CGAAAAT55900.08.440941
CGACCTA28850.08.4209318
CAACGAT50800.08.3995191
CTCGAAC56650.08.36904051
GTCGGTT49950.08.31646251
TCGGTTT69200.08.3151312
Produced by FastQC (version 0.11.3)