FastQCFastQC Report
Thu 8 Dec 2016
24_200214_1HS_GGCTAC_L004_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename24_200214_1HS_GGCTAC_L004_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38653519
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAT45850.012.0501331
CTCGGTT50850.09.6678661
CTCGTTT58150.09.6563881
CTCCGAT52600.09.5605791
TCGAATG41700.08.8663542
CGAATGT41650.08.7507053
CGACGAT23550.08.713961
CGAAAAT43550.08.492211
CTCGGAT73700.08.4451371
CTCAAAT130200.08.400321
CTCGACT31850.08.2840281
CTGGAAT180800.08.2819911
CGGGAAT41800.08.14641
CGGGGAT49750.08.1138141
CTCGATT30550.08.0460061
CGAAAAC34500.08.0399081
CGACCTA21550.08.0378488
CGGAAAT44950.07.92670151
CTGAAAT163700.07.9073011
CGCCGAT18850.07.8958281
Produced by FastQC (version 0.11.3)