FastQCFastQC Report
Thu 8 Dec 2016
24_200214_3HS_GTCCGC_L005_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename24_200214_3HS_GTCCGC_L005_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48524665
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAT63150.013.3103951
CTCGTTT84700.011.3073541
CTCCGAT74700.011.1920281
CTCAAAT175800.09.8189581
CGAATGT56450.09.6851483
CTCGGAT85200.09.4688861
CTCGATT36700.09.3332521
CGGAAAT58900.09.2970771
TCGAATG60050.09.2918782
CTGGAAT238250.09.1463761
CTTAGAT110950.08.9977081
CGCAAAT29350.08.9064651
CGATCTA14000.08.8386624
CTCGGTT66550.08.702431
CGACAAT35500.08.6965651
ACGTCCG31350.08.68393732
CTTGAAT159400.08.341021
CGCAATT35550.08.3039961
CCGGAAT62250.08.2537431
CCGAAAC41850.08.2385641
Produced by FastQC (version 0.11.3)