FastQCFastQC Report
Thu 8 Dec 2016
24_240214_3HS_CCGTCC_L008_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename24_240214_3HS_CCGTCC_L008_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44242588
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG70850.021.275313
CGTATGC78500.019.5893461
ATGCCGT94750.017.2616164
GCCGTCT109800.014.9128116
TGCCGTC113850.014.52380855
CCGTCTT119200.013.9254937
GTATGCC111600.013.6680472
CTCGAAT56600.012.4292071
CTCGTTT67200.010.7706281
CGTCTTC161700.010.55752858
GCTTGAA169500.09.95247116
CGACGAT28900.09.6745121
CTCCGAT62850.09.5788021
CGAATGT51450.09.4451613
CTCGGTT62800.09.3350991
TCGAATG51150.09.3246212
CCGCAAT26500.09.2744011
CTGGAAT209450.09.2581311
CGCGTAA5851.8189894E-129.25040151
CCGGTAT21400.09.1666521
Produced by FastQC (version 0.11.3)