FastQCFastQC Report
Thu 8 Dec 2016
24_60314_6HS_ACTTGA_L002_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename24_60314_6HS_ACTTGA_L002_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44069932
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATGT70700.015.5297293
CTCGAAT93650.013.9272471
CTCGTTT129400.013.9267631
TCGAATG80650.013.390612
ATTTAGC162500.011.8247011
TAGAGCG130450.011.78108915
TAGCTCA142650.011.1349184
CGACGAT28800.010.8721841
GTTCGAT49100.010.6897831
CTCGGTT65200.010.4686221
CGTTTAG37750.010.2512053
TCGTTTT101850.010.1615572
GCGCAAG153750.010.15552434
CGCTTGC157650.010.11851420
GTGATAT100200.010.1048410
CTAACGC28950.010.02560323
TATACGC29650.010.01691310
GAGCGCT158400.09.97126717
CCGGAAT95600.09.920161
GCAAGCG156550.09.85890330
Produced by FastQC (version 0.11.3)