FastQCFastQC Report
Thu 8 Dec 2016
30_200214_1HS_GTGGCC_L004_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename30_200214_1HS_GTGGCC_L004_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54718325
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAT76800.014.12402251
CGAATGT73850.012.0333253
CTCGTTT102200.011.9377071
TCGAATG73850.011.325682
CTCGGTT78900.09.8037371
CTCCGAT94100.09.7299581
GTCGGTT69100.09.5297021
CCGGAAT72850.09.3177591
TCGGTTA40000.09.2432512
CTCGATT38000.09.1392741
TTCGATT48300.09.1018232
CGACGAT29200.09.0360281
CGCCGAT21950.08.9383891
CTCGGAT104350.08.8606571
CGCAATT44350.08.7459981
CTCAAAT209900.08.6810741
CGGAAAT67500.08.6196661
CGGGAAT60100.08.5552931
CGACCTA42350.08.5520038
CGCCAAT33350.08.5201891
Produced by FastQC (version 0.11.3)