FastQCFastQC Report
Thu 8 Dec 2016
30_200214_3HS_CGTACG_L005_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename30_200214_3HS_CGTACG_L005_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53121053
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAT65600.011.6788361
CCGGAAT64500.010.1661791
CGAATGT56500.09.7561693
CTCGGAT97300.09.6571221
CGGGAAT59650.09.3684061
CTCGGTT72300.09.3499111
CTCGTTT81900.09.2994511
CGACGAT32850.08.9858751
CTGGAAT260300.08.8297921
CTCCGAT76200.08.7530861
CGACCTA31900.08.74523458
CTCGCAT40750.08.7368321
CGCGAAT19000.08.6575251
TCGAATG59200.08.5131062
CTCAAAT180100.08.4578021
CTTAGAT115200.08.35218051
CTCGACT45300.08.2074881
TTCGATT43600.08.1532792
CCGGGAT76050.08.148131
GTTCGAT39600.08.1370191
Produced by FastQC (version 0.11.3)