FastQCFastQC Report
Thu 8 Dec 2016
30_240214_3HS_ACTGAT_L007_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename30_240214_3HS_ACTGAT_L007_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47696766
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAT56650.011.8618941
CTCGTTT73650.010.2261311
CTCGGAT87250.09.6400891
CTCCGAT65300.09.2546341
CTGGAAT232000.09.0684141
CTCGATT37850.08.8172571
CTCGGTT68850.08.7119491
CTCAAAT162700.08.6899571
GTCGGTT49850.08.6850791
CCGGAAT57550.08.5809721
CGAATGT49950.08.5137923
CGCAATT28800.08.4560771
CGACAAT31150.08.324871
CTTAGAT109250.08.2974041
CTCAGAT202000.08.1044381
CGCAAAT27500.08.0358351
CGCGAAT15900.07.94197561
TCGGTTT72550.07.93961952
CTTGAAT154700.07.9149321
TCGAATG51450.07.87198542
Produced by FastQC (version 0.11.3)