FastQCFastQC Report
Thu 8 Dec 2016
30_60314_3HS_ATTCCT_L001_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename30_60314_3HS_ATTCCT_L001_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43024127
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAT73050.015.291591
CGAATGT61600.014.5014053
TCGAATG68100.013.5137842
CTCGTTT91650.011.8933471
GTTCGAT39150.010.2395151
TTCGATT42450.010.0180892
CCGGAAT70650.09.8500291
CTCCGAT78500.09.7257081
CTCGGTT60900.09.6519351
GTGATAT87450.09.44149910
ACGCCAC90800.09.44142155
CGACCTA40350.09.3669438
CGCAATT47800.09.2817431
CGATTGG36150.09.2741964
TCGGTTA34000.09.1989162
AAGACGC88850.09.1928452
CTCAAAT171800.09.1893771
CAAGACG90100.09.0484671
CTCGGAT86600.09.0240771
CGGAAAT62850.08.886641
Produced by FastQC (version 0.11.3)