FastQCFastQC Report
Thu 8 Dec 2016
30_60314_6HS_CGTACG_L002_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename30_60314_6HS_CGTACG_L002_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34400179
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAT46450.012.3666381
CGAATGT39650.010.27059653
CGGAAAT41250.09.8844341
CTCGTTT57950.09.8347711
CTCCGAT50950.09.5500751
TCGAATG43450.09.4759242
CTGGAAT166500.09.2542031
CCGGAAT43550.09.2072311
CTCGGTT49350.09.1751
CTCGATT26000.09.0973131
TACGTAA19700.08.79401736
CCGTACG19850.08.727550533
GTACGTA19750.08.65782335
CGCGAAT12500.08.6502451
CTCGGAT62850.08.5662311
CGATAAC15450.08.4566171
CGGGGAT45100.08.4412611
GTCGGTT38450.08.2607591
CGCCAAT21700.08.2009561
CGACGAT20350.08.0808241
Produced by FastQC (version 0.11.3)