FastQCFastQC Report
Wed 7 Dec 2016
30_69_blood.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename30_69_blood.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37355629
Sequences flagged as poor quality0
Sequence length50
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCGA22150.050.7301844
CGTATGC39500.035.22062744
CTCGTAT38400.029.50866142
TATCTCG45700.024.98344439
TCTCGTA46350.024.77264841
TCGTATG52200.022.07894143
GCGTTCG43400.021.88990443
GTCACGC55250.020.97311829
CGTCGAT38750.020.2322612
ACACGTC57600.019.70606813
GCGTCGA40500.019.4643911
GATCGGA58600.018.9428771
AGTGCGT51000.018.83897840
CGCCAAT60550.018.74884833
TCGTGCG46350.018.5412037
ATCTCGT61500.018.42482240
CGTGCGT43700.018.0883678
CACGTCT67400.016.87306614
ACGTCTG68250.016.60016315
CACACGT69000.016.57525312
Produced by FastQC (version 0.11.3)