FastQCFastQC Report
Wed 7 Dec 2016
30_70_blood.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename30_70_blood.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37305899
Sequences flagged as poor quality0
Sequence length50
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT14500.036.70181744
TATCTCG30400.022.85026741
CGTTCGA17800.022.20960844
TCTCGTA34800.020.33304643
CGCGTTT4300.018.573031
TCGTGCG30450.017.9918927
CGTCGAT28800.016.92568812
GCGTTCG30900.016.47636243
CGTGCGT30700.016.0889428
TCGCAAC52100.015.73421240
CGACGAT21800.015.6440821
GCGTCGA32350.015.33500711
ACACGTC44950.015.30690313
ATCTCGT46850.015.10334842
AGTGCGT34800.015.06350840
TGCGAGA33550.014.91712935
CGCATAT6850.014.810061
GCGTAGC40950.014.11598810
GATCGGA48450.013.2761451
TAAGTCG44650.013.14075620
Produced by FastQC (version 0.11.3)